• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

The parafibromin tumor suppressor protein is part of a human Paf1 complex.

Parafibromin, the product of the HRPT2 (hyperparathyroidism-jaw tumor syndrome 2) tumor suppressor gene, is the human homologue of yeast Cdc73, part of the yeast RNA polymerase II/Paf1 complex known to be important for histone modification and connections to posttranscriptional events. By purifying cellular parafibromin and characterizing its associated proteins, we have identified a human counterpart to the yeast Paf1 complex including homologs of Leo1, Paf1, and Ctr9. Like the yeast complex, the parafibromin complex associates with the nonphosphorylated and Ser2 and Ser5 phosphorylated forms of the RNA polymerase II large subunit. Immunofluorescence experiments show that parafibromin is a nuclear protein. In addition, cotransfection data suggest that parafibromin can interact with a histone methyltransferase complex that methylates histone H3 on lysine 4. Some mutant forms of parafibromin lack association with hPaf1 complex members and with the histone methyltransferase complex, suggesting that disruption of these complexes may correlate with the oncogenic process.

Pubmed ID: 15632063

Authors

  • Rozenblatt-Rosen O
  • Hughes CM
  • Nannepaga SJ
  • Shanmugam KS
  • Copeland TD
  • Guszczynski T
  • Resau JH
  • Meyerson M

Journal

Molecular and cellular biology

Publication Data

January 5, 2005

Associated Grants

None

Mesh Terms

  • Cell Cycle Proteins
  • Cell Line
  • Histone-Lysine N-Methyltransferase
  • Humans
  • Multiprotein Complexes
  • Nuclear Proteins
  • Protein Methyltransferases
  • Proteins
  • RNA Polymerase II
  • Saccharomyces cerevisiae Proteins
  • Transcriptional Elongation Factors
  • Tumor Suppressor Proteins