Androgen receptor coregulator ARA267-alpha interacts with death receptor-6 revealed by the yeast two-hybrid.
ARA267-alpha is a newly identified androgen receptor coactivator. In order to further elucidate its precise role in cells, using the ARA267-alpha fragment containing four PHD and one SET conserved domains as bait we revealed an ARA267-alpha-PHD-SET-interacting protein, death receptor-6 (DR6), in the yeast two-hybrid screening. DR6 is the member of TNF receptor family and has a death domain in its intracellular cytoplasmic portion (DR6cp) to mediate the cell apoptosis. The interaction between ARA267-alpha-PHD-SET and DR6cp was confirmed in vitro and in vivo. Our finding implied that androgen signaling pathway might cross talk with apoptosis signaling pathway through the interaction between ARA267-alpha and DR6.
Pubmed ID: 15623156 RIS Download
Animals | Blotting, Western | Cell Line, Tumor | Cloning, Molecular | DNA, Complementary | Escherichia coli | Humans | Immunoprecipitation | Intracellular Signaling Peptides and Proteins | Mice | Nuclear Proteins | Plasmids | Protein Binding | Protein Structure, Tertiary | Receptors, Androgen | Receptors, Tumor Necrosis Factor | Signal Transduction | Two-Hybrid System Techniques