• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Restricted inactivation of serum response factor to the cardiovascular system.

Serum response factor (SRF) directs programs of gene expression linked to growth and muscle differentiation. To investigate the role of SRF in cardiovascular development, we generated mice in which SRF is knocked out in >80% of cardiomyocytes and >50% of vascular smooth muscle cells (SMC) through SM22alpha-Cre-mediated excision of SRF's promoter and first exon. Mutant mice display vascular patterning, cardiac looping, and SRF-dependent gene expression through embryonic day (e)9.5. At e10.5, attenuation in cardiac trabeculation and compact layer expansion is noted, with an attendant decrease in vascular SMC recruitment to the dorsal aorta. Ultrastructurally, cardiac sarcomeres and Z disks are highly disorganized in mutant embryos. Moreover, SRF mutant mice exhibit vascular SMC lacking organizing actin/intermediate filament bundles. These structural defects in the heart and vasculature coincide with decreases in SRF-dependent gene expression, such that by e11.5, when mutant embryos succumb to death, no SRF-dependent mRNA expression is evident. These results suggest a vital role for SRF in contractile/cytoskeletal architecture necessary for the proper assembly and function of cardiomyocytes and vascular SMC.

Pubmed ID: 15569937


  • Miano JM
  • Ramanan N
  • Georger MA
  • de Mesy Bentley KL
  • Emerson RL
  • Balza RO
  • Xiao Q
  • Weiler H
  • Ginty DD
  • Misra RP


Proceedings of the National Academy of Sciences of the United States of America

Publication Data

December 7, 2004

Associated Grants

  • Agency: NHLBI NIH HHS, Id: HL-60655
  • Agency: NHLBI NIH HHS, Id: HL-62572
  • Agency: NHLBI NIH HHS, Id: HL-67272
  • Agency: NINDS NIH HHS, Id: NS34814

Mesh Terms

  • Animals
  • Cardiovascular Abnormalities
  • Cardiovascular System
  • Embryo, Mammalian
  • Fetal Growth Retardation
  • Gene Expression Regulation
  • Heart Defects, Congenital
  • Mice
  • Mice, Knockout
  • Microfilament Proteins
  • Muscle Proteins
  • Phenotype
  • Serum Response Factor