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Interaction of Fcp1 phosphatase with elongating RNA polymerase II holoenzyme, enzymatic mechanism of action, and genetic interaction with elongator.

Fcp1 de-phosphorylates the RNA polymerase II (RNAPII) C-terminal domain (CTD) in vitro, and mutation of the yeast FCP1 gene results in global transcription defects and increased CTD phosphorylation levels in vivo. Here we show that the Fcp1 protein associates with elongating RNAPII holoenzyme in vitro. Our data suggest that the association of Fcp1 with elongating polymerase results in CTD de-phosphorylation when the native ternary RNAPII0-DNA-RNA complex is disrupted. Surprisingly, highly purified yeast Fcp1 dephosphorylates serine 5 but not serine 2 of the RNAPII CTD repeat. Only free RNAPII0(Ser-5) and not RNAPII0-DNA-RNA ternary complexes act as a good substrate in the Fcp1 CTD de-phosphorylation reaction. In contrast, TFIIH CTD kinase has a pronounced preference for RNAPII incorporated into a ternary complex. Interestingly, the Fcp1 reaction mechanism appears to entail phosphoryl transfer from RNAPII0 directly to Fcp1. Elongator fails to affect the phosphatase activity of Fcp1 in vitro, but genetic evidence points to a functional overlap between Elongator and Fcp1 in vivo. Genetic interactions between Elongator and a number of other transcription factors are also reported. Together, these results shed new light on mechanisms that drive the transcription cycle and point to a role for Fcp1 in the recycling of RNAPII after dissociation from active genes.

Pubmed ID: 15563457


  • Kong SE
  • Kobor MS
  • Krogan NJ
  • Somesh BP
  • S√łgaard TM
  • Greenblatt JF
  • Svejstrup JQ


The Journal of biological chemistry

Publication Data

February 11, 2005

Associated Grants


Mesh Terms

  • Acetylation
  • Biotin
  • Blotting, Western
  • Chromatin
  • Chromatography, Gel
  • Electrophoresis, Polyacrylamide Gel
  • Histidine
  • Mutation
  • Phosphoprotein Phosphatases
  • Phosphoric Monoester Hydrolases
  • Phosphorylation
  • Protein Binding
  • Protein Conformation
  • Protein Structure, Tertiary
  • RNA
  • RNA Polymerase II
  • Saccharomyces cerevisiae
  • Serine
  • Silver Staining
  • Time Factors
  • Transcription Factor TFIIH
  • Transcription Factors, TFII
  • Transcription, Genetic