• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


T cell-specific inactivation of the interleukin 10 gene in mice results in enhanced T cell responses but normal innate responses to lipopolysaccharide or skin irritation.

Interleukin (IL)-10 is a regulator of inflammatory responses and is secreted by a variety of different cell types including T cells. T regulatory cells have been shown to suppress immune responses by IL-10-dependent, but also IL-10-independent, mechanisms. Herein, we address the role of T cell-derived IL-10 in mice with an inactivation of the IL-10 gene restricted to T cells generated by Cre/loxP-mediated targeting of the IL-10 gene. Splenocytes from this T cell-specific mutant secrete increased amounts of proinflammatory cytokines after activation in vitro compared with show enhanced contact hypersensitivity reactions, and succumb to severe immunopathology upon infection with Toxoplasma gondii. Despite intact IL-10 genes in other cell types, the dysregulation of T cell responses observed in the T cell-specific IL-10 mutant closely resembles the phenotype in complete IL-10 deficiency. However, in contrast to complete IL-10 deficiency, sensitivity to endotoxic shock and irritant responses of the skin are not enhanced in the T cell-specific IL-10 mutant. Our data highlight the importance of T cell-derived IL-10 in the regulation of T cell responses and demonstrate that endotoxic shock and the irritant response of the skin are controlled by IL-10 from other cell types.

Pubmed ID: 15534372


  • Roers A
  • Siewe L
  • Strittmatter E
  • Deckert M
  • Schlüter D
  • Stenzel W
  • Gruber AD
  • Krieg T
  • Rajewsky K
  • Müller W


The Journal of experimental medicine

Publication Data

November 15, 2004

Associated Grants


Mesh Terms

  • Animals
  • Gene Silencing
  • Histological Techniques
  • Immunity, Cellular
  • Immunity, Innate
  • Interleukin-10
  • Intestinal Mucosa
  • Lipopolysaccharides
  • Mice
  • Mice, Transgenic
  • Mutagenesis, Insertional
  • Skin Irritancy Tests
  • Spleen
  • T-Lymphocytes
  • Toxoplasma