Baf60c is essential for function of BAF chromatin remodelling complexes in heart development.
Tissue-specific transcription factors regulate several important aspects of embryonic development. They must function in the context of DNA assembled into the higher-order structure of chromatin. Enzymatic complexes such as the Swi/Snf-like BAF complexes remodel chromatin to allow the transcriptional machinery access to gene regulatory elements. Here we show that Smarcd3, encoding Baf60c, a subunit of the BAF complexes, is expressed specifically in the heart and somites in the early mouse embryo. Smarcd3 silencing by RNA interference in mouse embryos derived from embryonic stem cells causes defects in heart morphogenesis that reflect impaired expansion of the anterior/secondary heart field, and also results in abnormal cardiac and skeletal muscle differentiation. An intermediate reduction in Smarcd3 expression leads to defects in outflow tract remodelling reminiscent of human congenital heart defects. Baf60c overexpressed in cell culture can mediate interactions between cardiac transcription factors and the BAF complex ATPase Brg1, thereby potentiating the activation of target genes. These results reveal tissue-specific and dose-dependent roles for Baf60c in recruiting BAF chromatin remodelling complexes to heart-specific enhancers, providing a novel mechanism to ensure transcriptional regulation during organogenesis.
Pubmed ID: 15525990 RIS Download
Animals | Chromatin Assembly and Disassembly | Chromosomal Proteins, Non-Histone | DNA-Binding Proteins | GATA4 Transcription Factor | Gene Expression Regulation, Developmental | Heart | Heart Defects, Congenital | Homeobox Protein Nkx-2.5 | Homeodomain Proteins | Immunoprecipitation | In Situ Hybridization | Mice | Mice, Transgenic | Multiprotein Complexes | Muscle Proteins | Myocardium | Protein Subunits | RNA Interference | RNA, Messenger | Somites | T-Box Domain Proteins | Transcription Factors | Transcriptional Activation