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RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components.

Sequential events in macromolecular biosynthesis are often elegantly coordinated. The small ribosomal subunit (SSU) processome is a large ribonucleoprotein (RNP) required for processing of precursors to the small subunit RNA, the 18S, of the ribosome. We have found that a subcomplex of SSU processome proteins, the t-Utps, is also required for optimal rRNA transcription in vivo in the yeast Saccharomyces cerevisiae. The t-Utps are ribosomal chromatin (r-chromatin)-associated, and they exist in a complex in the absence of the U3 snoRNA. Transcription is required neither for the formation of the subcomplex nor for its r-chromatin association. The t-Utps are associated with the pre-18S rRNAs independent of the presence of the U3 snoRNA. This association may thus represent an early step in the formation of the SSU processome. Our results indicate that rRNA transcription and pre-rRNA processing are coordinated via specific components of the SSU processome.

Pubmed ID: 15489292

Authors

  • Gallagher JE
  • Dunbar DA
  • Granneman S
  • Mitchell BM
  • Osheim Y
  • Beyer AL
  • Baserga SJ

Journal

Genes & development

Publication Data

October 15, 2004

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM 52581

Mesh Terms

  • Blotting, Western
  • Chromatin
  • DNA Primers
  • Immunoprecipitation
  • Models, Genetic
  • Polymerase Chain Reaction
  • RNA Polymerase I
  • RNA Precursors
  • RNA Processing, Post-Transcriptional
  • RNA, Ribosomal, 18S
  • Ribonucleoproteins
  • Ribosomes
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription, Genetic