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RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion.

The small GTPase Rap1 induces integrin-mediated adhesion and changes in the actin cytoskeleton. The mechanisms that mediate these effects of Rap1 are poorly understood. We have identified RIAM as a Rap1-GTP-interacting adaptor molecule. RIAM defines a family of adaptor molecules that contain a RA-like (Ras association) domain, a PH (pleckstrin homology) domain, and various proline-rich motifs. RIAM also interacts with Profilin and Ena/VASP proteins, molecules that regulate actin dynamics. Overexpression of RIAM induced cell spreading and lamellipodia formation, changes that require actin polymerization. In contrast, RIAM knockdown cells had reduced content of polymerized actin. RIAM overexpression also induced integrin activation and cell adhesion. RIAM knockdown displaced Rap1-GTP from the plasma membrane and abrogated Rap1-induced adhesion. Thus, RIAM links Rap1 to integrin activation and plays a role in regulating actin dynamics.

Pubmed ID: 15469846


  • Lafuente EM
  • van Puijenbroek AA
  • Krause M
  • Carman CV
  • Freeman GJ
  • Berezovskaya A
  • Constantine E
  • Springer TA
  • Gertler FB
  • Boussiotis VA


Developmental cell

Publication Data

October 7, 2004

Associated Grants

  • Agency: NIAID NIH HHS, Id: AI 41584
  • Agency: NIAID NIH HHS, Id: AI 43552
  • Agency: NIAID NIH HHS, Id: AI 46584
  • Agency: NIGMS NIH HHS, Id: GM68676

Mesh Terms

  • Actins
  • Adaptor Proteins, Signal Transducing
  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Cell Adhesion
  • Cell Adhesion Molecules
  • Cell Line
  • Cercopithecus aethiops
  • Contractile Proteins
  • Glutathione Transferase
  • Humans
  • Integrins
  • Jurkat Cells
  • Ligands
  • Luciferases
  • Membrane Proteins
  • Microfilament Proteins
  • Molecular Sequence Data
  • Phosphoproteins
  • Profilins
  • Proline
  • Protein Conformation
  • Protein Structure, Tertiary
  • Pseudopodia
  • Recombinant Fusion Proteins
  • Sequence Homology, Amino Acid
  • rap1 GTP-Binding Proteins