• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts.

TPX2 has multiple functions during mitosis, including microtubule nucleation around the chromosomes and the targeting of Xklp2 and Aurora A to the spindle. We have performed a detailed domain functional analysis of TPX2 and found that a large N-terminal domain containing the Aurora A binding peptide interacts directly with and nucleates microtubules in pure tubulin solutions. However, it cannot substitute the endogenous TPX2 to support microtubule nucleation in response to Ran guanosine triphosphate (GTP) and spindle assembly in egg extracts. By contrast, a large C-terminal domain of TPX2 that does not bind directly to pure microtubules and does not bind Aurora A kinase rescues microtubule nucleation in response to RanGTP and spindle assembly in TPX2-depleted extract. These and previous results suggest that under physiological conditions, TPX2 is essential for microtubule nucleation around chromatin and functions in a network of other molecules, some of which also are regulated by RanGTP.

Pubmed ID: 15385625

Authors

  • Brunet S
  • Sardon T
  • Zimmerman T
  • Wittmann T
  • Pepperkok R
  • Karsenti E
  • Vernos I

Journal

Molecular biology of the cell

Publication Data

December 23, 2004

Associated Grants

None

Mesh Terms

  • Animals
  • Aurora Kinases
  • Cell Cycle Proteins
  • Cell Extracts
  • Cell Polarity
  • Enzyme Activation
  • Kinesin
  • Microtubule-Associated Proteins
  • Microtubules
  • Neoplasm Proteins
  • Nuclear Proteins
  • Oocytes
  • Phosphoproteins
  • Protein Kinases
  • Protein Structure, Tertiary
  • Protein-Serine-Threonine Kinases
  • Spindle Apparatus
  • Xenopus Proteins
  • Xenopus laevis