• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


SUZ12 is required for both the histone methyltransferase activity and the silencing function of the EED-EZH2 complex.

Recent studies have revealed the intrinsic histone methyltransferase (HMTase) activity of the EED-EZH2 complex and its role in Hox gene silencing, X inactivation, and cancer metastasis. In this study, we focus on the function of individual components. We found that the HMTase activity requires a minimum of three components-EZH2, EED, and SUZ12-while AEBP2 is required for optimal enzymatic activity. Using a stable SUZ12 knockdown cell line, we show SUZ12 knockdown results in cell growth defects, which correlate with genome-wide alteration on H3-K27 methylation as well as upregulation of a number of Hox genes. Chromatin immunoprecipitation (ChIP) assay identified a 500 bp region located 4 kb upstream of the HoxA9 transcription initiation site as a SUZ12 binding site, which responds to SUZ12 knockdown and might play an important role in regulating HoxA9 expression. Thus, our study establishes a critical role of SUZ12 in H3-lysine 27 methylation and Hox gene silencing.

Pubmed ID: 15225548


  • Cao R
  • Zhang Y


Molecular cell

Publication Data

July 2, 2004

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM68804

Mesh Terms

  • Binding Sites
  • Cell Division
  • DNA-Binding Proteins
  • Gene Expression Regulation, Developmental
  • Gene Silencing
  • Gene Targeting
  • Genes, Homeobox
  • HeLa Cells
  • Histone-Lysine N-Methyltransferase
  • Histones
  • Homeodomain Proteins
  • Humans
  • Lysine
  • Methylation
  • Mutation
  • Neoplasm Proteins
  • Polycomb Repressive Complex 2
  • Protein Methyltransferases
  • Proteins
  • Repressor Proteins
  • Silencer Elements, Transcriptional
  • Transcription Factors
  • Up-Regulation