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Identification of TFB5, a new component of general transcription and DNA repair factor IIH.

We previously described the use of quantitative proteomics to study macromolecular complexes. Applying the method to analyze a yeast RNA polymerase II preinitiation complex, we identified a new 8-kDa protein, encoded by the uncharacterized open reading frame YDR079c-a, as a potential new component of the preinitiation complex. Here we show that YDR079c-a is a bona fide component of polymerase II preinitiation complexes and investigate its role in transcription. YDR079c-a is recruited to promoters both in vivo and in vitro and is required for efficient transcription in vitro and for normal induction of GAL genes. In addition, YDR079c-a is a core component of general transcription and DNA repair factor IIH and is required for efficient recruitment of TFIIH to a promoter. Yeast lacking YDR079c-a grow slowly, and, like strains carrying mutations in core TFIIH subunits, are sensitive to ultraviolet radiation. YDR079c-a is conserved throughout evolution, and mutations in the human ortholog account for a DNA repair-deficient form of the tricothiodystrophy disorder called TTD-A(2). The identification of a new, evolutionarily conserved, core TFIIH subunit is essential for our understanding of TFIIH function in transcription, DNA repair and human disease.

Pubmed ID: 15220919

Authors

  • Ranish JA
  • Hahn S
  • Lu Y
  • Yi EC
  • Li XJ
  • Eng J
  • Aebersold R

Journal

Nature genetics

Publication Data

July 30, 2004

Associated Grants

None

Mesh Terms

  • DNA Polymerase II
  • DNA Repair
  • Plasmids
  • Saccharomyces cerevisiae
  • Transcription Factor TFIIH
  • Transcription Factors, TFII
  • Transcription, Genetic