Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Targeted mutagenesis of the murine IRP1 and IRP2 genes reveals context-dependent RNA processing differences in vivo.

RNA (New York, N.Y.) | Jul 21, 2004

http://www.ncbi.nlm.nih.gov/pubmed/15208438

We report the targeted mutagenesis of the murine iron regulatory protein (IRP)-1 and IRP2 genes, respectively, with a classical gene trap construct. Insertion of the targeting cassette into the second intron of either gene by homologous recombination interrupts their open reading frames near the N termini. Mice that are homozygous for the correctly modified IRP1 or IRP2 alleles, respectively, display a strong reduction (90%, IRP1(-/-)) or nondetectable levels (IRP2(-/-)) of the targeted proteins. Interestingly, the pre-mRNAs transcribed from the identical targeting cassettes are processed differently within the two different contexts. Detailed analysis of the respective products identifies the choice of alternative splice and 3' end processing sites in the same tissues in vivo. We discuss the implications for the understanding of RNA processing and for targeting strategies for functional genomics in the mouse.

Pubmed ID: 15208438 RIS Download

Mesh terms: Alternative Splicing | Animals | Base Sequence | DNA Primers | Exons | Genome | Iron Regulatory Protein 1 | Iron Regulatory Protein 2 | Mice | Mutagenesis, Site-Directed | RNA | RNA Precursors | RNA Processing, Post-Transcriptional | RNA, Messenger | Recombination, Genetic | Restriction Mapping | Reverse Transcriptase Polymerase Chain Reaction

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

None

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.