We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

The chromatin remodeler Mi-2beta is required for CD4 expression and T cell development.

Immunity | Jun 10, 2004

Changes in chromatin structure underlie the activation or silencing of genes during development. The chromatin remodeler Mi-2beta is highly expressed in thymocytes and is presumed to be a transcriptional repressor because of its presence in the nucleosome remodeling deacetylase (NuRD) complex. Using conditional inactivation, we show that Mi-2beta is required at several steps during T cell development: for differentiation of beta selected immature thymocytes, for developmental expression of CD4, and for cell divisions in mature T cells. We further show that Mi-2beta plays a direct role in promoting CD4 gene expression. Mi-2beta associates with the CD4 enhancer as well as the E box binding protein HEB and the histone acetyltransferase (HAT) p300, enabling their recruitment to the CD4 enhancer and causing histone H3-hyperacetylation to this regulatory region. These findings provide important insights into the regulation of CD4 expression during T cell development and define a role for Mi-2beta in gene activation.

Pubmed ID: 15189737 RIS Download

Mesh terms: Acetyltransferases | Animals | Antigens, CD4 | Apoptosis | Basic Helix-Loop-Helix Transcription Factors | Cell Cycle Proteins | Cell Differentiation | Cell Division | Cells, Cultured | Chromatin Assembly and Disassembly | DNA-Binding Proteins | Enhancer Elements, Genetic | Flow Cytometry | Gene Expression Regulation | Histone Acetyltransferases | Histone Deacetylases | Mi-2 Nucleosome Remodeling and Deacetylase Complex | Mice | Mice, Transgenic | Receptors, Antigen, T-Cell | T-Lymphocytes | Thymus Gland | Transcription Factors | Transcriptional Activation | Transgenes | p300-CBP Transcription Factors

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIAID NIH HHS, Id: R01 AI038342
  • Agency: NIAID NIH HHS, Id: R01-AI1380342-09

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.