• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


The chromatin remodeler Mi-2beta is required for CD4 expression and T cell development.

Changes in chromatin structure underlie the activation or silencing of genes during development. The chromatin remodeler Mi-2beta is highly expressed in thymocytes and is presumed to be a transcriptional repressor because of its presence in the nucleosome remodeling deacetylase (NuRD) complex. Using conditional inactivation, we show that Mi-2beta is required at several steps during T cell development: for differentiation of beta selected immature thymocytes, for developmental expression of CD4, and for cell divisions in mature T cells. We further show that Mi-2beta plays a direct role in promoting CD4 gene expression. Mi-2beta associates with the CD4 enhancer as well as the E box binding protein HEB and the histone acetyltransferase (HAT) p300, enabling their recruitment to the CD4 enhancer and causing histone H3-hyperacetylation to this regulatory region. These findings provide important insights into the regulation of CD4 expression during T cell development and define a role for Mi-2beta in gene activation.

Pubmed ID: 15189737


  • Williams CJ
  • Naito T
  • Arco PG
  • Seavitt JR
  • Cashman SM
  • De Souza B
  • Qi X
  • Keables P
  • Von Andrian UH
  • Georgopoulos K



Publication Data

June 10, 2004

Associated Grants

  • Agency: NIAID NIH HHS, Id: R01-AI1380342-09

Mesh Terms

  • Acetyltransferases
  • Animals
  • Antigens, CD4
  • Apoptosis
  • Basic Helix-Loop-Helix Transcription Factors
  • Cell Cycle Proteins
  • Cell Differentiation
  • Cell Division
  • Cells, Cultured
  • Chromatin Assembly and Disassembly
  • DNA-Binding Proteins
  • Enhancer Elements, Genetic
  • Flow Cytometry
  • Gene Expression Regulation
  • Histone Acetyltransferases
  • Histone Deacetylases
  • Mi-2 Nucleosome Remodeling and Deacetylase Complex
  • Mice
  • Mice, Transgenic
  • Receptors, Antigen, T-Cell
  • T-Lymphocytes
  • Thymus Gland
  • Transcription Factors
  • Transcriptional Activation
  • Transgenes
  • p300-CBP Transcription Factors