Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Recoverin regulates light-dependent phosphodiesterase activity in retinal rods.

http://www.ncbi.nlm.nih.gov/pubmed/15173221

The Ca2+-binding protein recoverin may regulate visual transduction in retinal rods and cones, but its functional role and mechanism of action remain controversial. We compared the photoresponses of rods from control mice and from mice in which the recoverin gene was knocked out. Our analysis indicates that Ca2+-recoverin prolongs the dark-adapted flash response and increases the rod's sensitivity to dim steady light. Knockout rods had faster Ca2+ dynamics, indicating that recoverin is a significant Ca2+ buffer in the outer segment, but incorporation of exogenous buffer did not restore wild-type behavior. We infer that Ca2+-recoverin potentiates light-triggered phosphodiesterase activity, probably by effectively prolonging the catalytic activity of photoexcited rhodopsin.

Pubmed ID: 15173221 RIS Download

Mesh terms: Adaptation, Physiological | Animals | Calcium Signaling | Calcium-Binding Proteins | Cells, Cultured | Dose-Response Relationship, Radiation | Enzyme Activation | Eye Proteins | Light | Lipoproteins | Membrane Potentials | Mice | Mice, Inbred C57BL | Mice, Knockout | Phosphoric Diester Hydrolases | Recombinant Proteins | Recoverin | Retinal Rod Photoreceptor Cells

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIA NIH HHS, Id: AG12288
  • Agency: NEI NIH HHS, Id: EY05750
  • Agency: NEI NIH HHS, Id: EY12703
  • Agency: NEI NIH HHS, Id: EY12944
  • Agency: NEI NIH HHS, Id: EY14047
  • Agency: NEI NIH HHS, Id: R01 EY014047
  • Agency: NEI NIH HHS, Id: R01 EY014047-04

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.