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Dissection of the mammalian midbody proteome reveals conserved cytokinesis mechanisms.

Cytokinesis is the essential process that partitions cellular contents into daughter cells. To identify and characterize cytokinesis proteins rapidly, we used a functional proteomic and comparative genomic strategy. Midbodies were isolated from mammalian cells, proteins were identified by multidimensional protein identification technology (MudPIT), and protein function was assessed in Caenorhabditis elegans. Of 172 homologs disrupted by RNA interference, 58% displayed defects in cleavage furrow formation or completion, or germline cytokinesis. Functional dissection of the midbody demonstrated the importance of lipid rafts and vesicle trafficking pathways in cytokinesis, and the utilization of common membrane cytoskeletal components in diverse morphogenetic events in the cleavage furrow, the germline, and neurons.

Pubmed ID: 15166316

Authors

  • Skop AR
  • Liu H
  • Yates J
  • Meyer BJ
  • Heald R

Journal

Science (New York, N.Y.)

Publication Data

July 2, 2004

Associated Grants

  • Agency: NIGMS NIH HHS, Id: F32 GM064159
  • Agency: NIGMS NIH HHS, Id: F32 GM064159-01
  • Agency: NIGMS NIH HHS, Id: F32 GM064159-02
  • Agency: NIGMS NIH HHS, Id: F32 GM064159-03
  • Agency: NIGMS NIH HHS, Id: F32 GM64159-01
  • Agency: NCRR NIH HHS, Id: P41 RR011823
  • Agency: NCRR NIH HHS, Id: RR1823

Mesh Terms

  • Animals
  • CHO Cells
  • Caenorhabditis elegans
  • Carrier Proteins
  • Cell Cycle
  • Cell Division
  • Cell Fractionation
  • Cell Membrane
  • Computational Biology
  • Cricetinae
  • Cytoskeletal Proteins
  • Cytoskeleton
  • Germ Cells
  • HeLa Cells
  • Humans
  • Membrane Microdomains
  • Morphogenesis
  • Organelles
  • Protein Transport
  • Proteins
  • Proteome
  • Proteomics
  • Signal Transduction
  • Spindle Apparatus