• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Ssu72 Is an RNA polymerase II CTD phosphatase.

Phosphorylation of serine-2 (S2) and serine-5 (S5) of the C-terminal domain (CTD) of RNA polymerase II (RNAP II) is a dynamic process that regulates the transcription cycle and coordinates recruitment of RNA processing factors. The Fcp1 CTD phosphatase catalyzes dephosphorylation of S2-P. Here, we report that Ssu72, a component of the yeast cleavage/polyadenylation factor (CPF) complex, is a CTD phosphatase with specificity for S5-P. Ssu72 catalyzes CTD S5-P dephosphorylation in association with the Pta1 component of the CPF complex, although its essential role in 3' end processing is independent of catalytic activity. Depletion of Ssu72 impairs transcription in vitro, and this defect can be rescued by recombinant, catalytically active Ssu72. We propose that Ssu72 has a dual role in transcription, one as a CTD S5-P phosphatase that regenerates the initiation-competent, hypophosphorylated form of RNAP II and the other as a factor necessary for cleavage of pre-mRNA and efficient transcription termination.

Pubmed ID: 15125841

Authors

  • Krishnamurthy S
  • He X
  • Reyes-Reyes M
  • Moore C
  • Hampsey M

Journal

Molecular cell

Publication Data

May 7, 2004

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM39484
  • Agency: NIGMS NIH HHS, Id: GM41752
  • Agency: NIGMS NIH HHS, Id: GM68887

Mesh Terms

  • Carrier Proteins
  • Genes, Regulator
  • Phosphoprotein Phosphatases
  • Protein Structure, Tertiary
  • RNA Polymerase II
  • RNA, Messenger
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Serine
  • Substrate Specificity
  • Transcription, Genetic
  • mRNA Cleavage and Polyadenylation Factors