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Gene-specific modulation of TAF10 function by SET9-mediated methylation.

Molecular cell | Apr 23, 2004

http://www.ncbi.nlm.nih.gov/pubmed/15099517

SET9 is a member of the SET domain-containing histone methyltransferase family that can specifically methylate histone 3 at lysine 4 position. Although nucleosomal histones are poor substrates for SET9, the active enzyme can stimulate activator-induced transcription. Here, we show that SET9 can monomethylate the TBP-associated factor TAF10 at a single lysine residue located at the loop 2 region within the putative histone-fold domain of the protein. Methylated TAF10 has an increased affinity for RNA polymerase II, pointing to a direct role of this modification in preinitiation complex formation. Reporter assays and studies on TAF10 null F9 cells expressing a methylation-deficient TAF10 mutant revealed that SET9-mediated methylation of TAF10 potentiates transcription of some but not all TAF10-dependent genes. This gene specificity correlated with SET9 recruitment. The promoter-specific effects of SET9-methylated TAF10 may have important implications regarding the biological function of SET domain-containing lysine methylases, whose primary targets have been presumed to be histones.

Pubmed ID: 15099517 RIS Download

Mesh terms: Amino Acid Sequence | Antibodies, Monoclonal | Baculoviridae | Binding Sites | Cell Line | Cell Nucleus | Escherichia coli | Gene Expression Regulation | Genes, Reporter | Genetic Vectors | HeLa Cells | Histones | Humans | Lysine | Methylation | Methyltransferases | Mutation | Nucleosomes | Precipitin Tests | Protein Structure, Tertiary | Proteins | RNA Polymerase II | Recombinant Fusion Proteins | Substrate Specificity | TATA-Binding Protein Associated Factors | Time Factors | Transcription Factor TFIID | Transcription, Genetic | Transcriptional Activation

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