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Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.

The anaphase-promoting complex (APC/C) is a large ubiquitin-protein ligase which controls progression through anaphase by triggering the degradation of cell cycle regulators such as securin and B-type cyclins. The APC/C is an unusually complex ligase containing at least 10 different, evolutionarily conserved components. In contrast to APC/C's role in cell cycle regulation little is known about the functions of individual subunits and how they might interact with each other. Here, we have analyzed Swm1/Apc13, a small subunit recently identified in the budding yeast complex. Database searches revealed proteins related to Swm1/Apc13 in various organisms including humans. Both the human and the fission yeast homologues are associated with APC/C subunits, and they complement the phenotype of an SWM1 deletion mutant of budding yeast. Swm1/Apc13 promotes the stable association with the APC/C of the essential subunits Cdc16 and Cdc27. Accordingly, Swm1/Apc13 is required for ubiquitin ligase activity in vitro and for the timely execution of APC/C-dependent cell cycle events in vivo.

Pubmed ID: 15060174

Authors

  • Schwickart M
  • Havlis J
  • Habermann B
  • Bogdanova A
  • Camasses A
  • Oelschlaegel T
  • Shevchenko A
  • Zachariae W

Journal

Molecular and cellular biology

Publication Data

April 2, 2004

Associated Grants

None

Mesh Terms

  • Amino Acid Sequence
  • Anaphase-Promoting Complex-Cyclosome
  • Animals
  • Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome
  • Cell Cycle
  • Cell Cycle Proteins
  • Chromatids
  • DNA Polymerase III
  • Evolution, Molecular
  • Genetic Complementation Test
  • Humans
  • Meiosis
  • Molecular Sequence Data
  • Protein Subunits
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Sequence Alignment
  • Ubiquitin-Protein Ligase Complexes
  • Ubiquitin-Protein Ligases