Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

TRAF7 potentiates MEKK3-induced AP1 and CHOP activation and induces apoptosis.

The tumor necrosis factor receptor-associated factor (TRAF) protein family members are critically involved in activation of NF-kappaB, JNK, and p38 activation triggered by tumor necrosis factor (TNF) receptor family members and toll/interleukin-1 receptor (TIR)-containing receptors. TRAF proteins (except for TRAF1) contain an N-terminal RING finger domain that is essential for their functions. In this report, we identified a protein designated as TRAF7, which contains a RING finger domain and a zinc finger domain that are mostly conserved with those of TRAFs. TRAF7 also contains seven WD40 repeats at its C terminus. TRAF7 specifically interacted with MEKK3 and potentiated MEKK3-mediated AP1 and CHOP activation. Depletion of TRAF7 by antisense RNA inhibited MEKK3-mediated AP1 and CHOP activation. Moreover, overexpression of TRAF7 induced caspase-dependent apoptosis. Domain mapping experiments indicated that TRAF7 potentiated MEKK3-mediated AP1 and CHOP activation and induced apoptosis through distinct domains. Our studies identified a novel TRAF family member that is involved in MEKK3 signaling and apoptosis.

Pubmed ID: 15001576


  • Xu LG
  • Li LY
  • Shu HB


The Journal of biological chemistry

Publication Data

April 23, 2004

Associated Grants


Mesh Terms

  • Amino Acid Sequence
  • Apoptosis
  • Blotting, Northern
  • Blotting, Western
  • CCAAT-Enhancer-Binding Proteins
  • Carrier Proteins
  • Caspases
  • Cell Death
  • DNA Fragmentation
  • Genes, Reporter
  • Humans
  • Luciferases
  • MAP Kinase Kinase Kinase 3
  • MAP Kinase Kinase Kinases
  • Mitogen-Activated Protein Kinases
  • Models, Genetic
  • Molecular Sequence Data
  • Plasmids
  • Precipitin Tests
  • Protein Structure, Tertiary
  • Receptors, Tumor Necrosis Factor
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Transcription Factor AP-1
  • Transcription Factor CHOP
  • Transcription Factors
  • Transfection
  • Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
  • Zinc Fingers
  • p38 Mitogen-Activated Protein Kinases