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PRDI-BF1 recruits the histone H3 methyltransferase G9a in transcriptional silencing.

Nature immunology | Mar 26, 2004

http://www.ncbi.nlm.nih.gov/pubmed/14985713

PRDI-BF1, the human ortholog of mouse Blimp-1, is a DNA-binding protein involved in postinduction repression of interferon-beta gene transcription in response to viral infection. PRDI-BF1 also has an essential function in driving terminal differentiation of B lymphocytes and therein silences multiple genes. Here we show PRDI-BF1 assembles silent chromatin over the interferon-beta promoter in the osteosarcoma cell line U2OS through recruitment of the histone H3 lysine methyltransferase G9a. G9a is recruited only when in a complex with PRDI-BF1. G9a catalytic activity is required for the accumulation of methylated histone H3 and transcriptional silencing mediated by PRDI-BF1 in vivo. This establishes a mechanism for the recruitment of G9a, the main mammalian euchromatic methyltransferase, and defines nonembryonic targets of G9a.

Pubmed ID: 14985713 RIS Download

Mesh terms: Amino Acid Sequence | Cell Line, Tumor | Chromatin | Gene Silencing | Histone-Lysine N-Methyltransferase | Humans | Interferon-beta | Macromolecular Substances | Methyltransferases | Molecular Sequence Data | Protein Methyltransferases | Protein Transport | Repressor Proteins | Substrate Specificity | Transcription Factors | Transcription, Genetic

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Associated grants

  • Agency: NCI NIH HHS, Id: CA 80990

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