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Dendrite development regulated by CREST, a calcium-regulated transcriptional activator.

The lasting effects of neuronal activity on brain development involve calcium-dependent gene expression. Using a strategy called transactivator trap, we cloned a calcium-responsive transactivator called CREST (for calcium-responsive transactivator). CREST is a SYT-related nuclear protein that interacts with adenosine 3',5'-monophosphate (cAMP) response element-binding protein (CREB)-binding protein (CBP) and is expressed in the developing brain. Mice that have a targeted disruption of the crest gene are viable but display defects in cortical and hippocampal dendrite development. Cortical neurons from crest mutant mice are compromised in calcium-dependent dendritic growth. Thus, calcium activation of CREST-mediated transcription helps regulate neuronal morphogenesis.

Pubmed ID: 14716005

Authors

  • Aizawa H
  • Hu SC
  • Bobb K
  • Balakrishnan K
  • Ince G
  • Gurevich I
  • Cowan M
  • Ghosh A

Journal

Science (New York, N.Y.)

Publication Data

January 9, 2004

Associated Grants

  • Agency: NIMH NIH HHS, Id: MH60598
  • Agency: NINDS NIH HHS, Id: NS39993

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Brain
  • CREB-Binding Protein
  • Calcium
  • Calcium Channels
  • Cell Line
  • Cells, Cultured
  • Cerebral Cortex
  • Cloning, Molecular
  • Dendrites
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Gene Library
  • Gene Targeting
  • Humans
  • In Situ Hybridization
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Mutation
  • Nervous System
  • Neurons
  • Nuclear Proteins
  • Protein Structure, Tertiary
  • Rats
  • Recombinant Fusion Proteins
  • Trans-Activators
  • Transcription, Genetic
  • Transcriptional Activation
  • Transfection