Dendrite development regulated by CREST, a calcium-regulated transcriptional activator.
The lasting effects of neuronal activity on brain development involve calcium-dependent gene expression. Using a strategy called transactivator trap, we cloned a calcium-responsive transactivator called CREST (for calcium-responsive transactivator). CREST is a SYT-related nuclear protein that interacts with adenosine 3',5'-monophosphate (cAMP) response element-binding protein (CREB)-binding protein (CBP) and is expressed in the developing brain. Mice that have a targeted disruption of the crest gene are viable but display defects in cortical and hippocampal dendrite development. Cortical neurons from crest mutant mice are compromised in calcium-dependent dendritic growth. Thus, calcium activation of CREST-mediated transcription helps regulate neuronal morphogenesis.
Pubmed ID: 14716005 RIS Download
Amino Acid Sequence | Animals | Blotting, Northern | Brain | CREB-Binding Protein | Calcium | Calcium Channels | Cell Line | Cells, Cultured | Cerebral Cortex | Cloning, Molecular | Dendrites | Gene Expression Profiling | Gene Expression Regulation, Developmental | Gene Library | Gene Targeting | Humans | In Situ Hybridization | Mice | Mice, Knockout | Molecular Sequence Data | Mutation | Nervous System | Neurons | Nuclear Proteins | Protein Structure, Tertiary | Rats | Recombinant Fusion Proteins | Trans-Activators | Transcription, Genetic | Transcriptional Activation | Transfection