Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Dendrite development regulated by CREST, a calcium-regulated transcriptional activator.

The lasting effects of neuronal activity on brain development involve calcium-dependent gene expression. Using a strategy called transactivator trap, we cloned a calcium-responsive transactivator called CREST (for calcium-responsive transactivator). CREST is a SYT-related nuclear protein that interacts with adenosine 3',5'-monophosphate (cAMP) response element-binding protein (CREB)-binding protein (CBP) and is expressed in the developing brain. Mice that have a targeted disruption of the crest gene are viable but display defects in cortical and hippocampal dendrite development. Cortical neurons from crest mutant mice are compromised in calcium-dependent dendritic growth. Thus, calcium activation of CREST-mediated transcription helps regulate neuronal morphogenesis.

Pubmed ID: 14716005


  • Aizawa H
  • Hu SC
  • Bobb K
  • Balakrishnan K
  • Ince G
  • Gurevich I
  • Cowan M
  • Ghosh A


Science (New York, N.Y.)

Publication Data

January 9, 2004

Associated Grants

  • Agency: NIMH NIH HHS, Id: MH60598
  • Agency: NINDS NIH HHS, Id: NS39993

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Brain
  • CREB-Binding Protein
  • Calcium
  • Calcium Channels
  • Cell Line
  • Cells, Cultured
  • Cerebral Cortex
  • Cloning, Molecular
  • Dendrites
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Gene Library
  • Gene Targeting
  • Humans
  • In Situ Hybridization
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Mutation
  • Nervous System
  • Neurons
  • Nuclear Proteins
  • Protein Structure, Tertiary
  • Rats
  • Recombinant Fusion Proteins
  • Trans-Activators
  • Transcription, Genetic
  • Transcriptional Activation
  • Transfection