Shifting foci of hematopoiesis during reconstitution from single stem cells.
To reveal the early events and dynamics of hematopoietic reconstitution in living animals in real-time, we used bioluminescence imaging to monitor engraftment from single luciferase-labeled hematopoietic stem cells (HSC) in irradiated recipients. Transplanted HSC generated discrete foci in the spleen and bone marrow (BM), at a frequency that correlated with BM compartment size. Initially detected foci could expand locally, seed other sites in BM or spleen, and/or recede with different kinetics. These studies reveal dynamic and variable patterns of engraftment from highly purified HSC and indicate that the final overall contribution of individual HSC to hematopoietic chimerism does not depend on the specific site of initial engraftment and expansion.
Proceedings of the National Academy of Sciences of the United States of America
January 6, 2004
Agency: NCI NIH HHS, Id: CA86065
Agency: NCI NIH HHS, Id: P01CA49605
Agency: NCI NIH HHS, Id: P20CA86312
Agency: NIDDK NIH HHS, Id: R01DK58664
Agency: NCI NIH HHS, Id: R24CA92862
Agency: NCI NIH HHS, Id: R33CA88303
Bone Marrow Cells
Green Fluorescent Proteins
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
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