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The gene product Murr1 restricts HIV-1 replication in resting CD4+ lymphocytes.

Although human immunodeficiency virus-1 (HIV-1) infects quiescent and proliferating CD4+ lymphocytes, the virus replicates poorly in resting T cells. Factors that block viral replication in these cells might help to prolong the asymptomatic phase of HIV infection; however, the molecular mechanisms that control this process are not fully understood. Here we show that Murr1, a gene product known previously for its involvement in copper regulation, inhibits HIV-1 growth in unstimulated CD4+ T cells. This inhibition was mediated in part through its ability to inhibit basal and cytokine-stimulated nuclear factor (NF)-kappaB activity. Knockdown of Murr1 increased NF-kappaB activity and decreased IkappaB-alpha concentrations by facilitating phospho-IkappaB-alpha degradation by the proteasome. Murr1 was detected in CD4+ T cells, and RNA-mediated interference of Murr1 in primary resting CD4+ lymphocytes increased HIV-1 replication. Through its effects on the proteasome, Murr1 acts as a genetic restriction factor that inhibits HIV-1 replication in lymphocytes, which could contribute to the regulation of asymptomatic HIV infection and the progression of AIDS.

Pubmed ID: 14685242

Authors

  • Ganesh L
  • Burstein E
  • Guha-Niyogi A
  • Louder MK
  • Mascola JR
  • Klomp LW
  • Wijmenga C
  • Duckett CS
  • Nabel GJ

Journal

Nature

Publication Data

December 18, 2003

Associated Grants

None

Mesh Terms

  • Adaptor Proteins, Signal Transducing
  • CD4-Positive T-Lymphocytes
  • Carrier Proteins
  • Cell Cycle
  • Cysteine Endopeptidases
  • HIV-1
  • Humans
  • I-kappa B Proteins
  • Jurkat Cells
  • Multienzyme Complexes
  • NF-kappa B
  • Proteasome Endopeptidase Complex
  • Proteins
  • RNA Interference
  • Virus Replication