A protein interaction map of Drosophila melanogaster.
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.
Pubmed ID: 14605208 RIS Download
Animals | Calcium | Cell Cycle | Cell Differentiation | Cloning, Molecular | Computational Biology | DNA, Complementary | Drosophila Proteins | Drosophila melanogaster | Genes, Insect | Immunity, Innate | Mathematics | Models, Statistical | Photoreceptor Cells, Invertebrate | Protein Binding | Protein Interaction Mapping | Proteome | RNA Splicing | RNA, Messenger | Receptor, Epidermal Growth Factor | Signal Transduction | Transcription, Genetic | Two-Hybrid System Techniques