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A protein interaction map of Drosophila melanogaster.

Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.

Pubmed ID: 14605208


  • Giot L
  • Bader JS
  • Brouwer C
  • Chaudhuri A
  • Kuang B
  • Li Y
  • Hao YL
  • Ooi CE
  • Godwin B
  • Vitols E
  • Vijayadamodar G
  • Pochart P
  • Machineni H
  • Welsh M
  • Kong Y
  • Zerhusen B
  • Malcolm R
  • Varrone Z
  • Collis A
  • Minto M
  • Burgess S
  • McDaniel L
  • Stimpson E
  • Spriggs F
  • Williams J
  • Neurath K
  • Ioime N
  • Agee M
  • Voss E
  • Furtak K
  • Renzulli R
  • Aanensen N
  • Carrolla S
  • Bickelhaupt E
  • Lazovatsky Y
  • DaSilva A
  • Zhong J
  • Stanyon CA
  • Finley RL
  • White KP
  • Braverman M
  • Jarvie T
  • Gold S
  • Leach M
  • Knight J
  • Shimkets RA
  • McKenna MP
  • Chant J
  • Rothberg JM


Science (New York, N.Y.)

Publication Data

December 5, 2003

Associated Grants

  • Agency: NHGRI NIH HHS, Id: HG01536

Mesh Terms

  • Animals
  • Calcium
  • Cell Cycle
  • Cell Differentiation
  • Cloning, Molecular
  • Computational Biology
  • DNA, Complementary
  • Drosophila Proteins
  • Drosophila melanogaster
  • Genes, Insect
  • Immunity, Innate
  • Mathematics
  • Models, Statistical
  • Photoreceptor Cells, Invertebrate
  • Protein Binding
  • Protein Interaction Mapping
  • Proteome
  • RNA Splicing
  • RNA, Messenger
  • Receptor, Epidermal Growth Factor
  • Signal Transduction
  • Transcription, Genetic
  • Two-Hybrid System Techniques