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Targets of the cyclin-dependent kinase Cdk1.

The events of cell reproduction are governed by oscillations in the activities of cyclin-dependent kinases (Cdks). Cdks control the cell cycle by catalysing the transfer of phosphate from ATP to specific protein substrates. Despite their importance in cell-cycle control, few Cdk substrates have been identified. Here, we screened a budding yeast proteomic library for proteins that are directly phosphorylated by Cdk1 in whole-cell extracts. We identified about 200 Cdk1 substrates, several of which are phosphorylated in vivo in a Cdk1-dependent manner. The identities of these substrates reveal that Cdk1 employs a global regulatory strategy involving phosphorylation of other regulatory molecules as well as phosphorylation of the molecular machines that drive cell-cycle events. Detailed analysis of these substrates is likely to yield important insights into cell-cycle regulation.

Pubmed ID: 14574415

Authors

  • Ubersax JA
  • Woodbury EL
  • Quang PN
  • Paraz M
  • Blethrow JD
  • Shah K
  • Shokat KM
  • Morgan DO

Journal

Nature

Publication Data

October 23, 2003

Associated Grants

None

Mesh Terms

  • Adenosine Triphosphate
  • CDC2 Protein Kinase
  • Cell Cycle
  • Consensus Sequence
  • Phosphorylation
  • Proteome
  • Reproducibility of Results
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Substrate Specificity