Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression.

Molecular cell | Aug 10, 2003

http://www.ncbi.nlm.nih.gov/pubmed/14536086

Methylation of histone tails plays an important role in chromatin structure and function. Previously, we reported that ESET/SETDB1 is a histone methyltransferase (HMTase). Here, we show that SETDB1 tightly associates with the human homolog of mAM, a murine ATFa-associated factor. Although recombinant ESET can methylate lysine 9 of histone H3 (H3-K9), its activity is severely compromised when compared to that of the ESET/mAM complex. mAM stimulates ESET enzymatic activity by increasing the Vmax and decreasing the Km. Importantly, mAM facilitates the ESET-dependent conversion of dimethyl H3-K9 to the trimethyl state both in vitro and in vivo. Chromatin-based transcription and ChIP analyses demonstrate that mAM enhances ESET-mediated transcriptional repression in a SAM-dependent manner, and this repression correlates with H3-K9 trimethylation at the promoter. Thus, our studies establish that promoter H3-K9 trimethylation is the cause of transcriptional repression and that mAM/hAM facilitates conversion of H3-K9 dimethyl to trimethyl by ESET/SETDB1.

Pubmed ID: 14536086 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Binding Sites | Blotting, Western | Cell Line | Chromatin | Dose-Response Relationship, Drug | Electrophoresis, Polyacrylamide Gel | Enzymes | HeLa Cells | Histone-Lysine N-Methyltransferase | Histones | Humans | Kinetics | Lysine | Methylation | Methyltransferases | Mice | Molecular Sequence Data | Precipitin Tests | Promoter Regions, Genetic | Protein Binding | Protein Methyltransferases | RNA Interference | Recombinant Proteins | Repressor Proteins | Silver Staining | Substrate Specificity | Time Factors | Transcription Factors | Transcription, Genetic

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.