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A 3' exonuclease that specifically interacts with the 3' end of histone mRNA.

Molecular cell | Aug 10, 2003

Metazoan histone mRNAs end in a highly conserved stem-loop structure followed by ACCCA. Previous studies have suggested that the stem-loop binding protein (SLBP) is the only protein binding this region. Using RNA affinity purification, we identified a second protein, designated 3'hExo, that contains a SAP and a 3' exonuclease domain and binds the same sequence. Strikingly, 3'hExo can bind the stem-loop region both separately and simultaneously with SLBP. Binding of 3'hExo requires the terminal ACCCA, whereas binding of SLBP requires the 5' side of the stem-loop region. Recombinant 3'hExo degrades RNA substrates in a 3'-5' direction and has the highest activity toward the wild-type histone mRNA. Binding of SLBP to the stem-loop at the 3' end of RNA prevents its degradation by 3'hExo. These features make 3'hExo a primary candidate for the exonuclease that initiates rapid decay of histone mRNA upon completion and/or inhibition of DNA replication.

Pubmed ID: 14536070 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Baculoviridae | Cloning, Molecular | DNA | Exonucleases | HeLa Cells | Histones | Humans | Mass Spectrometry | Models, Genetic | Molecular Sequence Data | Nuclear Proteins | Precipitin Tests | Protein Binding | Protein Structure, Tertiary | RNA | RNA, Messenger | RNA-Binding Proteins | Rabbits | Time Factors | Two-Hybrid System Techniques | mRNA Cleavage and Polyadenylation Factors

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