Our hosting provider is investigating network issues. We apologize for the inconvenience.

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

A 3' exonuclease that specifically interacts with the 3' end of histone mRNA.

Molecular cell | Aug 10, 2003

Metazoan histone mRNAs end in a highly conserved stem-loop structure followed by ACCCA. Previous studies have suggested that the stem-loop binding protein (SLBP) is the only protein binding this region. Using RNA affinity purification, we identified a second protein, designated 3'hExo, that contains a SAP and a 3' exonuclease domain and binds the same sequence. Strikingly, 3'hExo can bind the stem-loop region both separately and simultaneously with SLBP. Binding of 3'hExo requires the terminal ACCCA, whereas binding of SLBP requires the 5' side of the stem-loop region. Recombinant 3'hExo degrades RNA substrates in a 3'-5' direction and has the highest activity toward the wild-type histone mRNA. Binding of SLBP to the stem-loop at the 3' end of RNA prevents its degradation by 3'hExo. These features make 3'hExo a primary candidate for the exonuclease that initiates rapid decay of histone mRNA upon completion and/or inhibition of DNA replication.

Pubmed ID: 14536070 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Baculoviridae | Cloning, Molecular | DNA | Exonucleases | HeLa Cells | Histones | Humans | Mass Spectrometry | Models, Genetic | Molecular Sequence Data | Nuclear Proteins | Precipitin Tests | Protein Binding | Protein Structure, Tertiary | RNA | RNA, Messenger | RNA-Binding Proteins | Rabbits | Time Factors | Two-Hybrid System Techniques | mRNA Cleavage and Polyadenylation Factors

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: GM29832

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.