• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

RhoA/ROCK regulation of neuritogenesis via profilin IIa-mediated control of actin stability.

Neuritogenesis, the first step of neuronal differentiation, takes place as nascent neurites bud from the immediate postmitotic neuronal soma. Little is known about the mechanisms underlying the dramatic morphological changes that characterize this event. Here, we show that RhoA activity plays a decisive role during neuritogenesis of cultured hippocampal neurons by recruiting and activating its specific kinase ROCK, which, in turn, complexes with profilin IIa. We establish that this previously uncharacterized brain-specific actin-binding protein controls neurite sprouting by modifying actin stability, a function regulated by ROCK-mediated phosphorylation. Furthermore, we determine that this novel cascade is switched on or off by physiological stimuli. We propose that RhoA/ROCK/PIIa-mediated regulation of actin stability, shown to be essential for neuritogenesis, may constitute a central mechanism throughout neuronal differentiation.

Pubmed ID: 14517206

Authors

  • Da Silva JS
  • Medina M
  • Zuliani C
  • Di Nardo A
  • Witke W
  • Dotti CG

Journal

The Journal of cell biology

Publication Data

September 29, 2003

Associated Grants

None

Mesh Terms

  • Actin Cytoskeleton
  • Animals
  • Cell Size
  • Cells, Cultured
  • Contractile Proteins
  • Hippocampus
  • Intracellular Signaling Peptides and Proteins
  • Microfilament Proteins
  • Nerve Growth Factors
  • Neurites
  • Neurons
  • Profilins
  • Protein-Serine-Threonine Kinases
  • Rats
  • Signal Transduction
  • Stimulation, Chemical
  • rho-Associated Kinases
  • rhoA GTP-Binding Protein