Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

RhoA/ROCK regulation of neuritogenesis via profilin IIa-mediated control of actin stability.

http://www.ncbi.nlm.nih.gov/pubmed/14517206

Neuritogenesis, the first step of neuronal differentiation, takes place as nascent neurites bud from the immediate postmitotic neuronal soma. Little is known about the mechanisms underlying the dramatic morphological changes that characterize this event. Here, we show that RhoA activity plays a decisive role during neuritogenesis of cultured hippocampal neurons by recruiting and activating its specific kinase ROCK, which, in turn, complexes with profilin IIa. We establish that this previously uncharacterized brain-specific actin-binding protein controls neurite sprouting by modifying actin stability, a function regulated by ROCK-mediated phosphorylation. Furthermore, we determine that this novel cascade is switched on or off by physiological stimuli. We propose that RhoA/ROCK/PIIa-mediated regulation of actin stability, shown to be essential for neuritogenesis, may constitute a central mechanism throughout neuronal differentiation.

Pubmed ID: 14517206 RIS Download

Mesh terms: Actin Cytoskeleton | Animals | Cell Size | Cells, Cultured | Contractile Proteins | Hippocampus | Intracellular Signaling Peptides and Proteins | Microfilament Proteins | Nerve Growth Factors | Neurites | Neurons | Profilins | Protein-Serine-Threonine Kinases | Rats | Signal Transduction | Stimulation, Chemical | rho-Associated Kinases | rhoA GTP-Binding Protein

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

None

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.