We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Bidirectional transmembrane signaling by cytoplasmic domain separation in integrins.

Science (New York, N.Y.) | Sep 19, 2003

Although critical for development, immunity, wound healing, and metastasis, integrins represent one of the few classes of plasma membrane receptors for which the basic signaling mechanism remains a mystery. We investigated cytoplasmic conformational changes in the integrin LFA-1 (alphaLbeta2) in living cells by measuring fluorescence resonance energy transfer between cyan fluorescent protein-fused and yellow fluorescent protein-fused alphaL and beta2 cytoplasmic domains. In the resting state these domains were close to each other, but underwent significant spatial separation upon either intracellular activation of integrin adhesiveness (inside-out signaling) or ligand binding (outside-in signaling). Thus, bidirectional integrin signaling is accomplished by coupling extracellular conformational changes to an unclasping and separation of the alpha and beta cytoplasmic domains, a distinctive mechanism for transmitting information across the plasma membrane.

Pubmed ID: 14500982 RIS Download

Mesh terms: Antibodies, Monoclonal | Antigens, CD11a | Antigens, CD18 | Bacterial Proteins | Cell Adhesion | Cell Membrane | Chemokine CXCL12 | Chemokines, CXC | Cytoplasm | Dimerization | Fluorescence Resonance Energy Transfer | Green Fluorescent Proteins | Humans | Intercellular Adhesion Molecule-1 | Ligands | Luminescent Proteins | Lymphocyte Function-Associated Antigen-1 | Protein Conformation | Protein Structure, Tertiary | Receptors, CXCR4 | Recombinant Fusion Proteins | Signal Transduction | Talin | Transfection | Tumor Cells, Cultured

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.