We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Prohibitin induces the transcriptional activity of p53 and is exported from the nucleus upon apoptotic signaling.

Prohibitin, a potential tumor suppressor protein, has been shown to inhibit cell proliferation and repress E2F transcriptional activity. Though prohibitin has potent transcriptional functions in the nucleus, a mitochondrial role for prohibitin has also been proposed. Here we show that prohibitin is predominantly nuclear in two breast cancer cell lines where it co-localizes with E2F1 and p53. Upon apoptotic stimulation by camptothecin, prohibitin is exported to perinuclear regions where it localizes to mitochondria. The data presented here also show that prohibitin is capable of physically interacting with p53 in vivo and in vitro. Prohibitin was found to enhance p53-mediated transcriptional activity and cotransfection of an antisense prohibitin construct reduces p53-mediated transcriptional activation. Prohibitin appears to induce p53-mediated transcription by enhancing its recruitment to promoters, as detected by chromatin immunoprecipitation assays. These results suggest that prohibitin is capable of modulating Rb/E2F as well as p53 regulatory pathways.

Pubmed ID: 14500729 RIS Download

Mesh terms: Active Transport, Cell Nucleus | Antineoplastic Agents, Phytogenic | Apoptosis | Binding Sites | Blotting, Western | Camptothecin | Cell Cycle Proteins | Cell Division | Cell Line, Tumor | Cell Nucleus | Chromatin | Cytosol | DNA-Binding Proteins | E2F Transcription Factors | E2F1 Transcription Factor | Enzyme Inhibitors | Genes, Reporter | Glutathione Transferase | Humans | Microscopy, Fluorescence | Mitochondria | Nuclear Proteins | Oligonucleotides, Antisense | Precipitin Tests | Promoter Regions, Genetic | Protein Binding | Protein Structure, Tertiary | Proteins | Proto-Oncogene Proteins | Proto-Oncogene Proteins c-mdm2 | Repressor Proteins | Retinoblastoma Protein | Signal Transduction | Transcription Factors | Transcription, Genetic | Transcriptional Activation | Transfection | Tumor Suppressor Protein p53

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: CA77301

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.