• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Yeast two-hybrid screens imply involvement of Fanconi anemia proteins in transcription regulation, cell signaling, oxidative metabolism, and cellular transport.

Mutations in one of at least eight different genes cause bone marrow failure, chromosome instability, and predisposition to cancer associated with the rare genetic syndrome Fanconi anemia (FA). The cloning of seven genes has provided the tools to study the molecular pathway disrupted in Fanconi anemia patients. The structure of the genes and their gene products provided few clues to their functional role. We report here the use of 3 FA proteins, FANCA, FANCC, and FANCG, as "baits" in the hunt for interactors to obtain clues for FA protein functions. Using five different human cDNA libraries we screened 36.5x10(6) clones with the technique of the yeast two-hybrid system. We identified 69 proteins which have not previously been linked to the FA pathway as direct interactors of FANCA, FANCC, or FANCG. Most of these proteins are associated with four functional classes including transcription regulation (21 proteins), signaling (13 proteins), oxidative metabolism (10 proteins), and intracellular transport (11 proteins). Interaction with 6 proteins, DAXX, Ran, IkappaBgamma, USP14, and the previously reported SNX5 and FAZF, was additionally confirmed by coimmunoprecipitation and/or colocalization studies. Taken together, our data strongly support the hypothesis that FA proteins are functionally involved in several complex cellular pathways including transcription regulation, cell signaling, oxidative metabolism, and cellular transport.

Pubmed ID: 14499622


  • Reuter TY
  • Medhurst AL
  • Waisfisz Q
  • Zhi Y
  • Herterich S
  • Hoehn H
  • Gross HJ
  • Joenje H
  • Hoatlin ME
  • Mathew CG
  • Huber PA


Experimental cell research

Publication Data

October 1, 2003

Associated Grants

  • Agency: NHLBI NIH HHS, Id: HL56045

Mesh Terms

  • Cell Cycle Proteins
  • Cells, Cultured
  • DNA Mutational Analysis
  • DNA, Complementary
  • DNA-Binding Proteins
  • Escherichia coli
  • Fanconi Anemia
  • Fanconi Anemia Complementation Group A Protein
  • Fanconi Anemia Complementation Group C Protein
  • Fanconi Anemia Complementation Group G Protein
  • Fanconi Anemia Complementation Group Proteins
  • Genes, Regulator
  • Humans
  • Nuclear Proteins
  • Oxidative Phosphorylation
  • Protein Transport
  • Proteins
  • Saccharomyces cerevisiae
  • Signal Transduction
  • Two-Hybrid System Techniques