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Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex.

The mammalian ATM/PI 3-kinase-related TRRAP protein was previously found to be a component of a multi-protein histone acetyltransferase (HAT) complex containing the HAT TIP60. In this report, we identify a previously uncharacterized protein encoded by the FLJ10914 ORF, which we designate MRGBP, as a new component of the TRRAP/TIP60 HAT complex. In addition, through purification of MRGBP and its associated proteins from HeLa cell nuclear extracts, we identify the thyroid receptor coactivating protein (TRCp120), DMAP1, and the related MRG15 and MRGX proteins as MRGBP-associating proteins, and we present biochemical evidence that they are previously unrecognized components of the TRRAP/TIP60 HAT complex. Taken together, our findings shed new light on the structure and function of the mammalian TRRAP/TIP60 histone acetyltransferase complex.

Pubmed ID: 12963728


  • Cai Y
  • Jin J
  • Tomomori-Sato C
  • Sato S
  • Sorokina I
  • Parmely TJ
  • Conaway RC
  • Conaway JW


The Journal of biological chemistry

Publication Data

October 31, 2003

Associated Grants

  • Agency: NIGMS NIH HHS, Id: R37 GM41628

Mesh Terms

  • Acetyltransferases
  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • Cell Nucleus
  • Chromatography, High Pressure Liquid
  • HeLa Cells
  • Histone Acetyltransferases
  • Humans
  • Mass Spectrometry
  • Nuclear Proteins
  • Open Reading Frames
  • Protein Binding
  • Receptors, Thyroid Hormone
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors