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A novel and essential mechanism determining specificity and activity of protein phosphatase 2A (PP2A) in vivo.

Genes & development | Sep 1, 2003

http://www.ncbi.nlm.nih.gov/pubmed/12952889

Protein phosphatase 2A (PP2A) is an essential intracellular serine/threonine phosphatase containing a catalytic subunit that possesses the potential to dephosphorylate promiscuously tyrosine-phosphorylated substrates in vitro. How PP2A acquires its intracellular specificity and activity for serine/threonine-phosphorylated substrates is unknown. Here we report a novel and phylogenetically conserved mechanism to generate active phospho-serine/threonine-specific PP2A in vivo. Phosphotyrosyl phosphatase activator (PTPA), a protein of so far unknown intracellular function, is required for the biogenesis of active and specific PP2A. Deletion of the yeast PTPA homologs generated a PP2A catalytic subunit with a conformation different from the wild-type enzyme, as indicated by its altered substrate specificity, reduced protein stability, and metal dependence. Complementation and RNA-interference experiments showed that PTPA fulfills an essential function conserved from yeast to man.

Pubmed ID: 12952889 RIS Download

Mesh terms: 3T3 Cells | Animals | Apoptosis | Binding Sites | HeLa Cells | Humans | Metals | Mice | Mutation | Phosphoprotein Phosphatases | Protein Phosphatase 2 | RNA Interference | Substrate Specificity

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