Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Robo4 is a vascular-specific receptor that inhibits endothelial migration.

Developmental biology | Sep 1, 2003

Guidance and patterning of axons are orchestrated by cell-surface receptors and ligands that provide directional cues. Interactions between the Robo receptor and Slit ligand families of proteins initiate signaling cascades that repel axonal outgrowth. Although the vascular and nervous systems grow as parallel networks, the mechanisms by which the vascular endothelial cells are guided to their appropriate positions remain obscure. We have identified a putative Robo homologue, Robo4, based on its differential expression in mutant mice with defects in vascular sprouting. In contrast to known neuronal Robo family members, the arrangement of the extracellular domains of Robo4 diverges significantly from that of all other Robo family members. Moreover, Robo4 is specifically expressed in the vascular endothelium during murine embryonic development. We show that Robo4 binds Slit and inhibits cellular migration in a heterologous expression system, analogous to the role of known Robo receptors in the nervous system. Immunoprecipitation studies indicate that Robo4 binds to Mena, a known effector of Robo-Slit signaling. Finally, we show that Robo4 is the only Robo family member expressed in primary endothelial cells and that application of Slit inhibits their migration. These data demonstrate that Robo4 is a bona fide member of the Robo family and may provide a repulsive cue to migrating endothelial cells during vascular development.

Pubmed ID: 12941633 RIS Download

Mesh terms: Activin Receptors, Type I | Activin Receptors, Type II | Amino Acid Sequence | Animals | Cell Line | Cell Movement | Chromosome Mapping | Endothelium, Vascular | Gene Expression Regulation, Developmental | Humans | In Situ Hybridization | Intercellular Signaling Peptides and Proteins | Ligands | Mice | Mice, Knockout | Molecular Sequence Data | Nerve Tissue Proteins | Phylogeny | RNA, Messenger | Receptors, Immunologic | Sequence Homology, Amino Acid | Signal Transduction | Zebrafish

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NEI NIH HHS, Id: R01 EY014576-03
  • Agency: NEI NIH HHS, Id: R01 EY12873
  • Agency: NIGMS NIH HHS, Id: R01 GM070967-02
  • Agency: NHLBI NIH HHS, Id: R01 HL077671-03
  • Agency: NCI NIH HHS, Id: R01 CA114197-01A2
  • Agency: NIA NIH HHS, Id: R01 AG17518

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.