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Long-term hematopoietic stem cells require stromal cell-derived factor-1 for colonizing bone marrow during ontogeny.

The physiological role of SDF-1 on hematopoietic stem cells (HSCs) remains elusive. We show that colonization of bone marrow by HSCs in addition to myeloid cells is severely impaired in SDF-1(-/-) embryos by a long-term repopulation assay. Colonization of spleen by HSCs was also affected, but to a lesser extent. Enforced expression of SDF-1 under the control of vascular-specific Tie-2 regulatory sequences could completely rescue the reduction of HSCs but not myeloid cells in SDF-1(-/-) bone marrow. SDF-1 was detected in the vicinity of the vascular endothelial cells in fetal bone marrow. SDF-1 plays a critical role in colonization of bone marrow by HSCs and myeloid cells during ontogeny, and the mechanisms by which SDF-1 functions are distinct between HSCs and myeloid cells.

Pubmed ID: 12932359


  • Ara T
  • Tokoyoda K
  • Sugiyama T
  • Egawa T
  • Kawabata K
  • Nagasawa T



Publication Data

August 22, 2003

Associated Grants


Mesh Terms

  • Animals
  • Bone Marrow
  • Chemokine CXCL12
  • Chemokines, CXC
  • Endothelium, Vascular
  • Gene Expression
  • Genes, Regulator
  • Hematopoiesis
  • Hematopoietic Stem Cells
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Myeloid Cells
  • Receptor Protein-Tyrosine Kinases
  • Receptor, TIE-2