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Long-term hematopoietic stem cells require stromal cell-derived factor-1 for colonizing bone marrow during ontogeny.

Immunity | Aug 22, 2003

The physiological role of SDF-1 on hematopoietic stem cells (HSCs) remains elusive. We show that colonization of bone marrow by HSCs in addition to myeloid cells is severely impaired in SDF-1(-/-) embryos by a long-term repopulation assay. Colonization of spleen by HSCs was also affected, but to a lesser extent. Enforced expression of SDF-1 under the control of vascular-specific Tie-2 regulatory sequences could completely rescue the reduction of HSCs but not myeloid cells in SDF-1(-/-) bone marrow. SDF-1 was detected in the vicinity of the vascular endothelial cells in fetal bone marrow. SDF-1 plays a critical role in colonization of bone marrow by HSCs and myeloid cells during ontogeny, and the mechanisms by which SDF-1 functions are distinct between HSCs and myeloid cells.

Pubmed ID: 12932359 RIS Download

Mesh terms: Animals | Bone Marrow | Chemokine CXCL12 | Chemokines, CXC | Endothelium, Vascular | Gene Expression | Genes, Regulator | Hematopoiesis | Hematopoietic Stem Cells | Mice | Mice, Inbred C57BL | Mice, Knockout | Mice, Transgenic | Myeloid Cells | Receptor Protein-Tyrosine Kinases | Receptor, TIE-2