SciCrunch will be offline today beginning at 6:30 PM PDT for about 15 minutes.
  • Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Activated Notch1 prevents differentiation of pancreatic acinar cells and attenuate endocrine development.

Mice carrying loss-of-function mutations in certain Notch pathway genes display increased and accelerated pancreatic endocrine development, leading to depletion of precursor cells followed by pancreatic hypoplasia. Here, we have investigated the effect of expressing a constitutively active form of the Notch1 receptor (Notch1(ICD)) in the developing pancreas using the pdx1 promoter. At e10.5 to e12.5, we observe a disorganized pancreatic epithelium with reduced numbers of endocrine cells, confirming a repressive activity of Notch1 upon the early differentiation program. Subsequent branching morphogenesis is impaired and the pancreatic epithelium forms cyst-like structures with ductal phenotype containing a few endocrine cells but completely devoid of acinar cells. The endocrine cells that do form show abnormal expression of cell type-specific markers. Our observations show that sustained Notch1 signaling not only significantly represses endocrine development, but also fully prevents pancreatic exocrine development, suggesting that a possible role of Notch1 is to maintain the undifferentiated state of common pancreatic precursor cells.

Pubmed ID: 12921743


  • Hald J
  • Hjorth JP
  • German MS
  • Madsen OD
  • Serup P
  • Jensen J


Developmental biology

Publication Data

August 15, 2003

Associated Grants

  • Agency: NIDDK NIH HHS, Id: DK-55284

Mesh Terms

  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Biological Markers
  • Cell Differentiation
  • Cell Nucleus
  • Gene Expression Regulation, Developmental
  • Homeodomain Proteins
  • Islets of Langerhans
  • Membrane Proteins
  • Mice
  • Mice, Inbred Strains
  • Mice, Transgenic
  • Nerve Tissue Proteins
  • Pancreas
  • Rats
  • Receptor, Notch1
  • Receptors, Cell Surface
  • Signal Transduction
  • Trans-Activators
  • Transcription Factors
  • Transcriptional Activation