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Degradation of normal mRNA in the nucleus of Saccharomyces cerevisiae.

A nuclear mRNA degradation (DRN) system was identified from analysis of mRNA turnover rates in nup116-Delta strains of Saccharomyces cerevisiae lacking the ability to export all RNAs, including poly(A) mRNAs, at the restrictive temperature. Northern blotting, in situ hybridization, and blocking transcription with thiolutin in nup116-delta strains revealed a rapid degradation of mRNAs in the nucleus that was suppressed by the rrp6-delta, rai1-delta, and cbc1-delta deletions, but not by the upf1-delta deletion, suggesting that DRN requires Rrp6p, a 3'-to-5' nuclear exonuclease, the Rat1p, a 5'-to-3' nuclear exonuclease, and Cbc1p, a component of CBC, the nuclear cap binding complex, which may direct the mRNAs to the site of degradation. We propose that certain normal mRNAs retained in the nucleus are degraded by the DRN system, similar to degradation of transcripts with 3' end formation defects in certain mutants.

Pubmed ID: 12897126

Authors

  • Das B
  • Butler JS
  • Sherman F

Journal

Molecular and cellular biology

Publication Data

August 4, 2003

Associated Grants

  • Agency: NIGMS NIH HHS, Id: R01 GM12702
  • Agency: NIGMS NIH HHS, Id: R01 GM59898

Mesh Terms

  • Blotting, Northern
  • Cell Nucleus
  • Exoribonucleases
  • Exosome Multienzyme Ribonuclease Complex
  • Gene Deletion
  • Genotype
  • In Situ Hybridization
  • In Situ Hybridization, Fluorescence
  • Microscopy, Fluorescence
  • Mutation
  • Nuclear Pore Complex Proteins
  • Phenotype
  • Poly A
  • Protein Binding
  • RNA, Messenger
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Temperature
  • Time Factors
  • Transcription, Genetic