Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Deletion of the SNARE vti1b in mice results in the loss of a single SNARE partner, syntaxin 8.

http://www.ncbi.nlm.nih.gov/pubmed/12861006

SNARE proteins participate in recognition and fusion of membranes. A SNARE complex consisting of vti1b, syntaxin 8, syntaxin 7, and endobrevin/VAMP-8 which is required for fusion of late endosomes in vitro has been identified recently. Here, we generated mice deficient in vti1b to study the function of this protein in vivo. vti1b-deficient mice had reduced amounts of syntaxin 8 due to degradation of the syntaxin 8 protein, while the amounts of syntaxin 7 and endobrevin did not change. These data indicate that vti1b is specifically required for the stability of a single SNARE partner. vti1b-deficient mice were viable and fertile. Most vti1b-deficient mice were indistinguishable from wild-type mice and did not display defects in transport to the lysosome. However, 20% of the vti1b-deficient mice were smaller. Lysosomal degradation of an endocytosed protein was slightly delayed in hepatocytes derived from these mice. Multivesicular bodies and autophagic vacuoles accumulated in hepatocytes of some smaller vti1b-deficient mice. This suggests that other SNAREs can compensate for the reduction in syntaxin 8 and for the loss of vti1b in most mice even though vti1b shows only 30% amino acid identity with its closest relative.

Pubmed ID: 12861006 RIS Download

Mesh terms: Alleles | Animals | Blotting, Northern | Blotting, Western | Carrier Proteins | Cells, Cultured | DNA, Complementary | Endosomes | Exons | Gene Library | Genetic Vectors | Genotype | Hepatocytes | Liver | Lysosomes | Membrane Proteins | Mice | Mice, Transgenic | Microscopy, Electron | Models, Genetic | Phenotype | Precipitin Tests | Qa-SNARE Proteins | Qb-SNARE Proteins | Qc-SNARE Proteins | R-SNARE Proteins | RNA, Messenger | Recombination, Genetic | SNARE Proteins | Time Factors | Vesicular Transport Proteins

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

None

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.