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An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid.

Nature immunology | Jul 27, 2003

http://www.ncbi.nlm.nih.gov/pubmed/12796777

Nucleotide-binding oligomerization domain protein 1 (NOD1) belongs to a family that includes multiple members with NOD and leucine-rich repeats in vertebrates and plants. NOD1 has been suggested to have a role in innate immune responses, but the mechanism involved remains unknown. Here we report that NOD1 mediates the recognition of peptidoglycan derived primarily from Gram-negative bacteria. Biochemical and functional analyses using highly purified and synthetic compounds indicate that the core structure recognized by NOD1 is a dipeptide, gamma-D-glutamyl-meso-diaminopimelic acid (iE-DAP). Murine macrophages deficient in NOD1 did not secrete cytokines in response to synthetic iE-DAP and did not prime the lipopolysaccharide response. Thus, NOD1 mediates selective recognition of bacteria through detection of iE-DAP-containing peptidoglycan.

Pubmed ID: 12796777 RIS Download

Mesh terms: Adaptor Proteins, Signal Transducing | Animals | Bacterial Infections | Carrier Proteins | Cell Line | Cytokines | Diaminopimelic Acid | Female | Humans | Immunity, Innate | Intracellular Signaling Peptides and Proteins | Lipopolysaccharides | Mice | Mice, Inbred C57BL | Nod1 Signaling Adaptor Protein | Nod2 Signaling Adaptor Protein | Peptidoglycan