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The role of yeast DNA 3'-phosphatase Tpp1 and rad1/Rad10 endonuclease in processing spontaneous and induced base lesions.

Tpp1 is a DNA 3'-phosphatase in Saccharomyces cerevisiae that is believed to act during strand break repair. It is homologous to one domain of mammalian polynucleotide kinase/3'-phosphatase. Unlike in yeast, we found that Tpp1 could confer resistance to methylmethane sulfonate when expressed in bacteria that lack abasic endonuclease/3'-phosphodiesterase function. This species difference was due to the absence of delta-lyase activity in S. cerevisiae, since expression of bacterial Fpg conferred Tpp1-dependent resistance to methylmethane sulfonate in yeast lacking the abasic endonucleases Apn1 and Apn2. In contrast, beta-only lyases increased methylmethane sulfonate sensitivity independently of Tpp1, which was explained by the inability of Tpp1 to cleave 3' alpha,beta-unsaturated aldehydes. In parallel experiments, mutations of TPP1 and RAD1, encoding part of the Rad1/Rad10 3'-flap endonuclease, caused synthetic growth defects in yeast strains lacking Apn1. In contrast, Fpg expression led to a partial rescue of apn1 apn2 rad1 synthetic lethality by converting lesions into Tpp1-cleavable 3'-phosphates. The collected experiments reveal a profound toxicity of strand breaks with irreparable 3' blocking lesions, and extend the function of the Rad1/Rad10 salvage pathway to 3'-phosphates. They further demonstrate a role for Tpp1 in repairing endogenously created 3'-phosphates. The source of these phosphates remains enigmatic, however, because apn1 tpp1 rad1 slow growth could be correlated with neither the presence of a yeast delta-lyase, the activity of the 3'-phosphate-generating enzyme Tdp1, nor levels of endogenous oxidation.

Pubmed ID: 12783866


  • Karumbati AS
  • Deshpande RA
  • Jilani A
  • Vance JR
  • Ramotar D
  • Wilson TE


The Journal of biological chemistry

Publication Data

August 15, 2003

Associated Grants

  • Agency: NHLBI NIH HHS, Id: 5T32HL07157
  • Agency: NCI NIH HHS, Id: CA-90911

Mesh Terms

  • Aldehydes
  • Aspartic Acid
  • DNA Damage
  • DNA Repair
  • DNA Repair Enzymes
  • DNA-Binding Proteins
  • DNA-Formamidopyrimidine Glycosylase
  • Deoxyribonuclease (Pyrimidine Dimer)
  • Endodeoxyribonucleases
  • Endonucleases
  • Escherichia coli Proteins
  • Fungal Proteins
  • Hydrogen Peroxide
  • Methyl Methanesulfonate
  • Mutagens
  • Mutation
  • N-Glycosyl Hydrolases
  • Nucleotidases
  • Oxidants
  • Phenotype
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Single-Strand Specific DNA and RNA Endonucleases