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phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development.

In this study, we utilize transgenic zebrafish with fluorescently labeled blood vessels to identify and characterize a mutant (y10) that displays specific defects in the formation of arteries, but not veins. We find that y10 encodes phospholipase C gamma-1 (plcg1), a known effector of receptor tyrosine kinase signaling. We further show that plcg1y10 mutant embryos fail to respond to exogenous Vegf. Our results indicate that Plcg1 functions specifically downstream of the Vegf receptor during embryonic development to govern formation of the arterial system.

Pubmed ID: 12782653

Authors

  • Lawson ND
  • Mugford JW
  • Diamond BA
  • Weinstein BM

Journal

Genes & development

Publication Data

June 1, 2003

Associated Grants

None

Mesh Terms

  • Animals
  • Animals, Genetically Modified
  • Arteries
  • Base Sequence
  • Crosses, Genetic
  • DNA Primers
  • Embryo, Nonmammalian
  • Endothelial Growth Factors
  • Female
  • Gene Expression Regulation, Developmental
  • Heterozygote Detection
  • Intercellular Signaling Peptides and Proteins
  • Lymphokines
  • Male
  • Molecular Sequence Data
  • Muscle, Smooth, Vascular
  • Phospholipase C gamma
  • Polymerase Chain Reaction
  • RNA, Messenger
  • Recombinant Proteins
  • Type C Phospholipases
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
  • Zebrafish