Alpha v beta 5 integrin-dependent programmed cell death triggered by a peptide mimic of annexin V.
The diverse cytoplasmic domain sequences within the various integrin subunits are critical for integrin-mediated signaling into the cell (outside-in signaling) and for activation of ligand binding affinity (inside-out signaling). Here we introduce an approach based on phage display technology to identify molecules that specifically interact with the cytoplasmic domain of the beta 5 integrin subunit. We show that a peptide selected for binding specifically to the beta 5 cytoplasmic domain (VVISYSMPD) induces apoptosis upon internalization. The cell death process induced by VVISYSMPD is sensitive to modulation by growth factors and by protein kinase C (PKC), and it cannot be triggered in beta 5 null cells. Finally, we show that the VVISYSMPD peptide is a mimic of annexin V. Our results suggest a functional link between the alpha v beta 5 integrin, annexin V, and programmed cell death. We propose the term "endothanatos" to designate this phenomenon.
Pubmed ID: 12769841 RIS Download
Amino Acid Sequence | Annexin A5 | Apoptosis | Cell Adhesion | Cell Line | Cell Membrane | Eukaryotic Cells | Genetic Vectors | Growth Substances | Humans | Integrins | Molecular Mimicry | Molecular Sequence Data | Peptides | Protein Kinase C | Protein Structure, Tertiary | Receptors, Vitronectin | Signal Transduction