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NEDP1, a highly conserved cysteine protease that deNEDDylates Cullins.

The ubiquitin-like protein NEDD8 is essential for activity of SCF-like ubiquitin ligase complexes. Here we identify and characterize NEDP1, a human NEDD8-specific protease. NEDP1 is highly conserved throughout evolution and equivalent proteins are present in yeast, plants, insects, and mammals. Bacterially expressed NEDP1 is capable of processing NEDD8 in vitro to expose the diglycine motif required for conjugation and can deconjugate NEDD8 from modified substrates. NEDP1 appears to be specific for NEDD8 as neither ubiquitin nor SUMO bearing COOH-terminal extensions are utilized as substrates. Inhibition studies and mutagenesis indicate that NEDP1 is a cysteine protease with sequence similarities to SUMO-specific proteases and the class of viral proteases typified by the adenovirus protease. In vivo NEDP1 deconjugates NEDD8 from a wide variety of substrates including the cullin component of SCF-like complexes. Thus NEDP1 is likely to play an important role in ubiquitin-mediated proteolysis by controlling the activity of SCF complexes.

Pubmed ID: 12730221


  • Mendoza HM
  • Shen LN
  • Botting C
  • Lewis A
  • Chen J
  • Ink B
  • Hay RT


The Journal of biological chemistry

Publication Data

July 11, 2003

Associated Grants


Mesh Terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Western
  • COS Cells
  • Cell Cycle Proteins
  • Cloning, Molecular
  • Cullin Proteins
  • Cysteine Endopeptidases
  • Dose-Response Relationship, Drug
  • Endopeptidases
  • Genetic Vectors
  • Glutathione Transferase
  • Humans
  • Mass Spectrometry
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Plasmids
  • Protein Binding
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins
  • Sequence Homology, Amino Acid
  • Substrate Specificity
  • Tissue Distribution
  • Transfection
  • Ubiquitin
  • Ubiquitins