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BIR-1, a Caenorhabditis elegans homologue of Survivin, regulates transcription and development.

bir-1, a Caenorhabditis elegans inhibitor-of-apoptosis gene homologous to Survivin is organized in an operon with the transcription cofactor C. elegans SKIP (skp-1). Because genes arranged in operons are frequently linked functionally, we have asked whether BIR-1 also functions in transcription. bir-1 inhibition resulted in multiple developmental defects that overlapped with C. elegans SKIP loss-of-function phenotypes: retention of eggs, dumpy, movement defects, and lethality. bir-1 RNA-mediated interference decreased expression of several gfp transgenes and the endogenous genes dpy-7 and hlh-1. Immunoblot analysis revealed decreased phosphoacetylated histones in bir-1 RNA-mediated interference-treated worms. In a heterologous transfection system, BIR-1 augments thyroid hormone-regulated transcription and has an additive effect with SKIP. These results show that BIR-1 functions in the regulation of transcription and development.

Pubmed ID: 12682297

Authors

  • Kostrouchova M
  • Kostrouch Z
  • Saudek V
  • Piatigorsky J
  • Rall JE

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

April 29, 2003

Associated Grants

None

Mesh Terms

  • Acetylation
  • Animals
  • Apoptosis
  • Blotting, Western
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins
  • Cell Division
  • Cell Line
  • Gene Expression Regulation, Developmental
  • Histones
  • Humans
  • Immunohistochemistry
  • RNA Interference
  • Thyroid Hormones
  • Transcription, Genetic