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ASAP, a novel protein complex involved in RNA processing and apoptosis.

Different isoforms of a protein complex termed the apoptosis- and splicing-associated protein (ASAP) were isolated from HeLa cell extract. ASAP complexes are composed of the polypeptides SAP18 and RNPS1 and different isoforms of the Acinus protein. While Acinus had previously been implicated in apoptosis and was recently identified as a component of the spliceosome, RNPS1 has been described as a general activator of RNA processing. Addition of ASAP isoforms to in vitro splicing reactions inhibits RNA processing mediated by ASF/SF2, by SC35, or by RNPS1. Additionally, microinjection of ASAP complexes into mammalian cells resulted in acceleration of cell death. Importantly, after induction of apoptosis the ASAP complex disassembles. Taken together, our results suggest an important role for the ASAP complexes in linking RNA processing and apoptosis.

Pubmed ID: 12665594


  • Schwerk C
  • Prasad J
  • Degenhardt K
  • Erdjument-Bromage H
  • White E
  • Tempst P
  • Kidd VJ
  • Manley JL
  • Lahti JM
  • Reinberg D


Molecular and cellular biology

Publication Data

April 31, 2003

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM 37120
  • Agency: NIGMS NIH HHS, Id: GM44088
  • Agency: NCI NIH HHS, Id: P30 CA08748
  • Agency: NCI NIH HHS, Id: P30 CA21765
  • Agency: NCI NIH HHS, Id: R01 CA53370
  • Agency: NIGMS NIH HHS, Id: R37 GM48259

Mesh Terms

  • Apoptosis
  • Carrier Proteins
  • HeLa Cells
  • Humans
  • In Vitro Techniques
  • Macromolecular Substances
  • Nuclear Proteins
  • Protein Isoforms
  • RNA Processing, Post-Transcriptional
  • RNA Splicing
  • Ribonucleoproteins
  • Transcription Factors