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Golgin-84 is a rab1 binding partner involved in Golgi structure.

Members of the golgin family of coiled-coil proteins have been implicated in the tethering of vesicles to Golgi membranes and cisternal membranes to each other. Many also bind to rab GTPases. Golgin-84 is a membrane-anchored golgin that we now show binds preferentially to the GTP form of the rab1 GTPase. It is also present throughout the Golgi stack by immuno-EM. Antibodies to golgin-84 inhibit stacking of cisternal membranes in a cell-free assay for Golgi reassembly, whereas the cytoplasmic domain of golgin-84 stimulates stacking and increases the length of re-assembled stacks. Transient expression of golgin-84 in NRK cells helps prevent the disassembly of the Golgi apparatus normally triggered by treatment with brefeldin A. Together these data suggest that golgin-84 is involved in generating and maintaining the architecture of the Golgi apparatus.

Pubmed ID: 12656988

Authors

  • Satoh A
  • Wang Y
  • Malsam J
  • Beard MB
  • Warren G

Journal

Traffic (Copenhagen, Denmark)

Publication Data

March 26, 2003

Associated Grants

  • Agency: NIGMS NIH HHS, Id: R01 GM087364
  • Agency: NIGMS NIH HHS, Id: R01 GM087364-01
  • Agency: NIGMS NIH HHS, Id: R01 GM087364-02
  • Agency: NIGMS NIH HHS, Id: R01 GM087364-03

Mesh Terms

  • Animals
  • Cells, Cultured
  • Cryoelectron Microscopy
  • Golgi Apparatus
  • Liver
  • Membrane Proteins
  • Microscopy, Immunoelectron
  • Mitosis
  • Molecular Sequence Data
  • Protein Binding
  • Rats
  • rab1 GTP-Binding Proteins