Enhanced interleukin (IL)-13 responses in mice lacking IL-13 receptor alpha 2.
Interleukin (IL)-13 has recently been shown to play important and unique roles in asthma, parasite immunity, and tumor recurrence. At least two distinct receptor components, IL-4 receptor (R)alpha and IL-13Ralpha1, mediate the diverse actions of IL-13. We have recently described an additional high affinity receptor for IL-13, IL-13Ralpha2, whose function in IL-13 signaling is unknown. To better appreciate the functional importance of IL-13Ralpha2, mice deficient in IL-13Ralpha2 were generated by gene targeting. Serum immunoglobulin E levels were increased in IL-13Ralpha2-/- mice despite the fact that serum IL-13 was absent and immune interferon gamma production increased compared with wild-type mice. IL-13Ralpha2-deficient mice display increased bone marrow macrophage progenitor frequency and decreased tissue macrophage nitric oxide and IL-12 production in response to lipopolysaccharide. These results are consistent with a phenotype of enhanced IL-13 responsiveness and demonstrate a role for endogenous IL-13 and IL-13Ralpha2 in regulating immune responses in wild-type mice.
Pubmed ID: 12642602 RIS Download
Animals | Cells, Cultured | Fibroblasts | Gene Targeting | Immunoglobulins | Interferon-gamma | Interleukin-13 | Interleukin-13 Receptor alpha1 Subunit | Macrophages | Male | Mice | Mice, Inbred BALB C | Mice, Inbred C57BL | Mice, Knockout | Receptors, Interleukin | Receptors, Interleukin-13 | Receptors, Interleukin-4 | STAT6 Transcription Factor | Signal Transduction | Stem Cells | Trans-Activators