We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Purification and functional characterization of the human N-CoR complex: the roles of HDAC3, TBL1 and TBLR1.

The EMBO journal | Mar 17, 2003

Corepressors N-CoR and SMRT participate in diverse repression pathways and exist in large protein complexes including HDAC3, TBL1 and TBLR1. However, the roles of these proteins in SMRT-N-CoR complex function are largely unknown. Here we report the purification and functional characterization of the human N-CoR complex. The purified N-CoR complex contains 10-12 associated proteins, including previously identified components and a novel actin-binding protein IR10. We show that TBL1/TBLR1 associates with N-CoR through two independent interactions: the N-terminal region and the C-terminal WD-40 repeats interact with the N-CoR RD1 and RD4 region, respectively. In vitro, TBL1/TBLR1 bind histones H2B and H4, and, importantly, repression by TBL1/TBLR1 correlates with their interaction with histones. By using specific small interference RNAs (siRNAs), we demonstrate that HDAC3 is essential, whereas TBL1 and TBLR1 are functionally redundant but essential for repression by unliganded thyroid hormone receptor. Together, our data reveal the roles of HDAC3 and TBL/TBLR1 and provide evidence for the functional importance of histone interaction in repression mediated by SMRT-N-CoR complexes.

Pubmed ID: 12628926 RIS Download

Mesh terms: Amino Acid Sequence | Cell Line | DNA-Binding Proteins | HeLa Cells | Histone Deacetylases | Histones | Humans | Microfilament Proteins | Nuclear Proteins | Peptide Fragments | Protein Binding | Protein Structure, Tertiary | Protein Subunits | RNA, Small Interfering | Receptors, Cytoplasmic and Nuclear | Receptors, Thyroid Hormone | Recombinant Proteins | Repressor Proteins | Sequence Homology, Amino Acid | Transducin

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIDDK NIH HHS, Id: R01 DK056324
  • Agency: NIDDK NIH HHS, Id: R01 DK058679
  • Agency: NIDDK NIH HHS, Id: DK56324
  • Agency: NIDDK NIH HHS, Id: DK58679

BioGRID (Data, Interactions)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.