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Microarray analysis of rat chromosome 2 congenic strains.

Human essential hypertension is a complex polygenic trait with underlying genetic components that remain unknown. The stroke-prone spontaneously hypertensive rat (SHRSP) is a model of human essential hypertension, and a number of reproducible blood pressure regulation quantitative trait loci have been found to map to rat chromosome 2. The SP.WKYGla2c* congenic strain was produced by introgressing a region of rat chromosome 2 from the normotensive Wistar Kyoto (WKY) strain into the genetic background of the SHRSP. Systolic and diastolic blood pressures were significantly reduced in the SP.WKYGla2c* compared with the SHRSP parental strain (198/134+/-6.1/3.3 versus 172/120+/-3.8/3.4 mm Hg; F=15.8/8.1, P=0.0009/0.013). Genome-wide microarray expression profiling was undertaken to identify differentially expressed genes among the parental SHRSP, WKY, and congenic strain. We identified a significant reduction in expression of glutathione S-transferase mu-type 2, a gene involved in the defense against oxidative stress. Quantitative reverse transcription-polymerase chain reaction relative to a beta-actin standard confirmed the microarray results with SHRSP mRNA at 8.56 x 10(-4) +/-1.6 x 10(-4) compared with SP.WKYGla2c* 3.67 x 10(-3)+/-2.8 x 10(-4) (95% CI -3.9 x 10(-3) to -1.8 x 10(-3); P=0.0034) and WKY 4.03 x 10(-3)+/-5.1 x 10(-4); (95% CI -5.4 x 10(-3) to -8.9 x 10(-4); P=0.027). We also identified regions of conserved synteny, each containing the Gstm2 gene, on mouse chromosome 3 and human chromosome 1.

Pubmed ID: 12624007


  • McBride MW
  • Carr FJ
  • Graham D
  • Anderson NH
  • Clark JS
  • Lee WK
  • Charchar FJ
  • Brosnan MJ
  • Dominiczak AF



Publication Data

March 7, 2003

Associated Grants


Mesh Terms

  • Animals
  • Animals, Congenic
  • Blood Pressure
  • Chromosome Mapping
  • Chromosomes, Mammalian
  • Gene Expression Profiling
  • Genome
  • Glutathione Transferase
  • Humans
  • Hypertension
  • Mice
  • Oligonucleotide Array Sequence Analysis
  • Phenotype
  • Quantitative Trait Loci
  • Radiation Hybrid Mapping
  • Rats
  • Rats, Inbred SHR
  • Rats, Inbred WKY
  • Synteny